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petN-psbM

LSC · 29523-30639 · 1117 bp

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Pi 0.00179

MarkerSeek score 59.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1117
variable_sites: 2
pi: 0.00179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	AGTTCCTACCGCTTTTCTACT	50.0	42.9	58.0	56.4	1206 bp	1.0	59.2
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	AGTTCCTACCGCTTTTCTACT	50.0	42.9	58.0	56.4	1204 bp	1.0	59.2
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	AGTTCCTACCGCTTTTCTACT	45.0	42.9	58.0	56.4	1251 bp	1.0	59.2
petN-psbM_p4	CGTAGTGTGGGGAAGAAGTG	AGTTCCTACCGCTTTTCTACT	55.0	42.9	57.9	56.4	1203 bp	1.0	58.6
petN-psbM_p5	AGTAAGTCTTGCTTGGGCTG	AGTTCCTACCGCTTTTCTACT	50.0	42.9	57.8	56.4	1257 bp	1.0	57.7