Hotspot

trnK-UUU-rps16

LSC · 4362-5059 · 698 bp

Back to Petunia

Pi 0.00575

MarkerSeek score 62.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 698
variable_sites: 4
pi: 0.00575
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TTGAAAAAGGGGCTCAACCT	50.0	45.0	58.2	57.5	829-830 bp	1.0	75.5
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TTGAAAAAGGGGCTCAACCT	50.0	45.0	58.2	57.5	828-829 bp	1.0	75.5
trnK-UUU-rps16_p3	TCAGTCGTGGTCTTCCAAAC	TTGAAAAAGGGGCTCAACCT	50.0	45.0	57.8	57.5	914-915 bp	1.0	74.9
trnK-UUU-rps16_p4	CAGTCGTGGTCTTCCAAACT	TTGAAAAAGGGGCTCAACCT	50.0	45.0	57.8	57.5	913-914 bp	1.0	74.8
trnK-UUU-rps16_p5	CCGCACTTAAAAGCCGAGTA	TTGAAAAAGGGGCTCAACCT	50.0	45.0	58.3	57.5	838-839 bp	1.0	74.5