Hotspot

ycf1

SSC · 119145-124706 · 5562 bp

Back to Petrorhagia

Pi 0.0225

MarkerSeek score 53.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5562
variable_sites: 124
pi: 0.0225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGGGTTTTCAGAATTCTTTGT	GGAAAACCTGGTTAAAATTAAAA	33.3	26.1	53.5	52.0	203-209 bp	1.0	43.9
ycf1_p2	AAGGGTTTTCAGAATTCTTTGT	GGAAAACCTGGTTAAAATTAAAA	31.8	26.1	54.4	52.0	204-210 bp	1.0	43.9
ycf1_p3	AGGGTTTTCAGAATTCTTTGT	GGAAAACCTGGTTAAAATTAAAAA	33.3	25.0	53.5	52.9	203-209 bp	1.0	43.9
ycf1_p4	AAGGGTTTTCAGAATTCTTTGT	GGAAAACCTGGTTAAAATTAAAAA	31.8	25.0	54.4	52.9	204-210 bp	1.0	43.9
ycf1_p5	AAAGGGTTTTCAGAATTCTTTGT	GGAAAACCTGGTTAAAATTAAAA	30.4	26.1	55.1	52.0	205-211 bp	1.0	43.8