Hotspot

ndhF

SSC · 105845-108097 · 2253 bp

Back to Petrorhagia

Pi 0.02

MarkerSeek score 54.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2253
variable_sites: 45
pi: 0.02
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGAGTCCATTTTCTGGGGAA	ACCTTTTAACGCCGACCAAT	45.0	45.0	56.3	58.1	2863 bp	0.5	45.9
ndhF_p2	AAAAATGCTTTTCGCCCTCT	TCTAGACGCGATCCTCTTGA	40.0	50.0	56.5	57.7	2855 bp	0.5	45.4
ndhF_p3	TGAGTCCATTTTCTGGGGAA	CGCCGACCAATACCCTTTAT	45.0	50.0	56.3	57.8	2854 bp	0.5	44.7
ndhF_p4	TGAGTCCATTTTCTGGGGAA	TTTAACGCCGACCAATACCC	45.0	50.0	56.3	58.3	2859 bp	0.5	44.6
ndhF_p5	TGAGTCCATTTTCTGGGGAA	AACCTTTTAACGCCGACCAA	45.0	45.0	56.3	58.3	2864 bp	0.5	44.1