Hotspot

psaC-ndhE

SSC · 112836-113211 · 376 bp

Back to Petrorhagia

Pi 0.0404

MarkerSeek score 76.10

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 376
variable_sites: 15
pi: 0.0404
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GCCTTACATCCATCCCAAGG	AAGCAGCTATTGGACTTGCT	55.0	45.0	58.0	57.5	567-572 bp	1.0	78.7
psaC-ndhE_p2	TCTAAGACATCCGTGGGACA	AAGCAGCTATTGGACTTGCT	50.0	45.0	57.8	57.5	540-545 bp	1.0	77.1
psaC-ndhE_p3	GCCTTACATCCATCCCAAGG	GAAGCAGCTATTGGACTTGC	55.0	50.0	58.0	57.2	568-573 bp	1.0	76.0
psaC-ndhE_p4	GGGACAAGCTCGAACACATT	AAGCAGCTATTGGACTTGCT	50.0	45.0	58.5	57.5	526-531 bp	1.0	75.2
psaC-ndhE_p5	TCTAAGACATCCGTGGGACA	GAAGCAGCTATTGGACTTGC	50.0	50.0	57.8	57.2	541-546 bp	1.0	74.4