Hotspot

rpl32-trnL-UAG

SSC · 108678-109565 · 888 bp

Back to Petrorhagia

Pi 0.0327

MarkerSeek score 64.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 888
variable_sites: 29
pi: 0.0327
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ATTGGTCGGCGTTAAAAGGT	ATCCGCATAAAAGAGCTGCA	45.0	45.0	58.1	58.0	1678-1699 bp	1.0	81.7
rpl32-trnL-UAG_p2	ATTGGTCGGCGTTAAAAGGT	TCCGCATAAAAGAGCTGCAT	45.0	45.0	58.1	58.0	1677-1698 bp	1.0	81.7
rpl32-trnL-UAG_p3	ATTGGTCGGCGTTAAAAGGT	GAGCTGCATAACCCAGTACC	45.0	55.0	58.1	58.0	1666-1687 bp	1.0	81.7
rpl32-trnL-UAG_p4	ATTGGTCGGCGTTAAAAGGT	AAGGCCTGAAGTAGCAAACC	45.0	50.0	58.1	58.1	1569-1590 bp	1.0	81.5
rpl32-trnL-UAG_p5	CCGGAAAAAGGAGAAGTCCC	TCCGCATAAAAGAGCTGCAT	55.0	45.0	58.2	58.0	2262-2465 bp	1.0	81.1