Hotspot

rpl22-rps19

LSC · 81129-81226 · 98 bp

Back to Petrorhagia

Pi 0.103

MarkerSeek score 71.80

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 98
variable_sites: 8
pi: 0.103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TATGCGTGCAATTCCTTCGA	AAGCTTAACACAAAGGCGGA	45.0	45.0	58.0	58.0	962-982 bp	1.0	82.5
rpl22-rps19_p2	TATGCGTGCAATTCCTTCGA	AGCTTAACACAAAGGCGGAA	45.0	45.0	58.0	58.0	961-981 bp	1.0	82.5
rpl22-rps19_p3	TATGCGTGCAATTCCTTCGA	TAGTAACTTGGTCTCGGGCA	45.0	50.0	58.0	58.1	931-951 bp	1.0	82.2
rpl22-rps19_p4	TATGCGTGCAATTCCTTCGA	TGGGTAGAGCCGGATCTAAG	45.0	55.0	58.0	58.0	1301-1321 bp	1.0	82.0
rpl22-rps19_p5	TATGCGTGCAATTCCTTCGA	AAAAGTTTGGGTAGAGCCGG	45.0	50.0	58.0	58.1	1308-1328 bp	1.0	81.2