Hotspot

psbE-petL

LSC · 62123-63285 · 1163 bp

Back to Petrorhagia

Pi 0.0254

MarkerSeek score 74.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1163
variable_sites: 27
pi: 0.0254
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GGAAGTTGTAGTTAAAGCCGC	50.0	47.6	57.9	57.5	1229-1324 bp	1.0	73.5
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GGAAGTTGTAGTTAAAGCCGC	50.0	47.6	57.8	57.5	1152-1247 bp	1.0	72.8
psbE-petL_p3	ACGAATAACCAACCCGCAAT	GGAAGTTGTAGTTAAAGCCGC	45.0	47.6	58.2	57.5	1238-1333 bp	1.0	72.6
psbE-petL_p4	TGCTGACGAATAACCAACCC	GGAAGTTGTAGTTAAAGCCGC	50.0	47.6	58.2	57.5	1243-1338 bp	1.0	72.5
psbE-petL_p5	CGAATAACCAACCCGCAATG	GGAAGTTGTAGTTAAAGCCGC	50.0	47.6	57.8	57.5	1237-1332 bp	1.0	72.4