Hotspot

ndhK-ndhC

LSC · 48531-48569 · 39 bp

Back to Petrorhagia

Pi 0.0769

MarkerSeek score 71.60

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 39
variable_sites: 3
pi: 0.0769
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	AGTGGAAACTCAATGGAATTCA	TGCATGGCTAAAAGGAGCTT	36.4	45.0	55.9	57.8	96 bp	1.0	47.9
ndhK-ndhC_p2	AGTGGAAACTCAATGGAATTCA	ATGCATGGCTAAAAGGAGCT	36.4	45.0	55.9	57.6	97 bp	1.0	46.0
ndhK-ndhC_p3	AGTAGTGGAAACTCAATGGA	TGCATGGCTAAAAGGAGCTT	40.0	45.0	53.6	57.8	99 bp	1.0	45.2
ndhK-ndhC_p4	AGTAGTGGAAACTCAATGGAA	TGCATGGCTAAAAGGAGCTT	38.1	45.0	54.5	57.8	99 bp	1.0	44.4
ndhK-ndhC_p5	AGTGGAAACTCAATGGAATTCAT	TGCATGGCTAAAAGGAGCTT	34.8	45.0	56.4	57.8	96 bp	1.0	44.0