Hotspot

ycf3-trnS-GGA

LSC · 42770-43328 · 559 bp

Back to Petrorhagia

Pi 0.0281

MarkerSeek score 75.00

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 559
variable_sites: 14
pi: 0.0281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGCCTCTTTTTCTCCCGAAG	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.8	58.1	672-732 bp	1.0	83.4
ycf3-trnS-GGA_p2	GCCTCTTTTTCTCCCGAAGT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.8	58.1	671-731 bp	1.0	83.4
ycf3-trnS-GGA_p3	TGCCTCTTTTTCTCCCGAAG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.8	58.2	693-753 bp	1.0	82.9
ycf3-trnS-GGA_p4	GCCTCTTTTTCTCCCGAAGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.8	58.2	692-752 bp	1.0	82.8
ycf3-trnS-GGA_p5	TGCCTCTTTTTCTCCCGAAG	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.8	57.8	643-703 bp	1.0	82.6