Hotspot

psaA-ycf3

LSC · 39585-40679 · 1095 bp

Back to Petrorhagia

Pi 0.0311

MarkerSeek score 68.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 1095
variable_sites: 32
pi: 0.0311
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTTCTTGAAAAATGGCCGGG	TTGGTTGAAGATCACGGGAC	50.0	50.0	57.9	57.8	1164-1230 bp	1.0	84.1
psaA-ycf3_p2	TTGAAAAATGGCCGGGTTTG	TTGGTTGAAGATCACGGGAC	45.0	50.0	57.8	57.8	1160-1226 bp	1.0	82.7
psaA-ycf3_p3	ACGACGTTTTTACGGGATCC	TTGGTTGAAGATCACGGGAC	50.0	50.0	58.3	57.8	1127-1193 bp	1.0	82.5
psaA-ycf3_p4	TCTTGAAAAATGGCCGGGTT	TTGGTTGAAGATCACGGGAC	45.0	50.0	58.3	57.8	1162-1228 bp	1.0	82.4
psaA-ycf3_p5	TTCTTGAAAAATGGCCGGGT	TTGGTTGAAGATCACGGGAC	45.0	50.0	58.3	57.8	1163-1229 bp	1.0	82.4