Hotspot

psaB-psaA

LSC · 37316-37331 · 16 bp

Back to Petrorhagia

Pi 0.0625

MarkerSeek score 82.80

Primer available yes

Label psaB-psaA

Feature Metadata

label_name: psaB-psaA
feature_type: igs
region: LSC
length_bp: 16
variable_sites: 1
pi: 0.0625
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB-psaA_p1	GGGTCCTGAGCTAAACCTTG	ACAACATGGGCGTTCTTCTT	55.0	45.0	57.9	58.0	108 bp	1.0	79.7
psaB-psaA_p2	GGTCCTGAGCTAAACCTTGG	ACAACATGGGCGTTCTTCTT	55.0	45.0	57.9	58.0	107 bp	1.0	79.7
psaB-psaA_p3	GGGTCCTGAGCTAAACCTTG	GTACAAGGACGTGCTGTAGG	55.0	55.0	57.9	58.0	159 bp	1.0	79.5
psaB-psaA_p4	GGTCCTGAGCTAAACCTTGG	GTACAAGGACGTGCTGTAGG	55.0	55.0	57.9	58.0	158 bp	1.0	79.5
psaB-psaA_p5	GTAGTGGGGTCCTGAGCTAA	GTACAAGGACGTGCTGTAGG	55.0	55.0	58.1	58.0	165 bp	1.0	79.3