Hotspot

trnR-UCU-atpA

LSC · 9474-9584 · 111 bp

Back to Petrorhagia

Pi 0.0659
MarkerSeek score 72.60
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
111
variable_sites
6
pi
0.0659
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.82
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 GGTTCAAATCCTATTGGACGC TGGAACGTTTTCTACTTCAGGA 47.6 40.9 57.3 57.3 145-165 bp 1.0 52.1
trnR-UCU-atpA_p2 GGTTCAAATCCTATTGGACGC TGGAACGTTTTCTACTTCAGGAA 47.6 39.1 57.3 58.0 145-165 bp 1.0 49.3
trnR-UCU-atpA_p3 TGGATAGGACAGAGGTCTTCT TGGAACGTTTTCTACTTCAGGA 47.6 40.9 56.9 57.3 180-200 bp 1.0 48.7
trnR-UCU-atpA_p4 GGTTCAAATCCTATTGGACGC TGGAACGTTTTCTACTTCAGG 47.6 42.9 57.3 55.8 145-165 bp 1.0 48.3
trnR-UCU-atpA_p5 GGAATGAAATGAAAAGCGTCCA TGGAACGTTTTCTACTTCAGGA 40.9 40.9 57.8 57.3 210-230 bp 1.0 47.4