Hotspot

trnS-GCU-trnG-UCC

LSC · 7670-8480 · 811 bp

Back to Petrorhagia

Pi 0.0668

MarkerSeek score 82.40

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 811
variable_sites: 52
pi: 0.0668
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CACGGAAAGAGAGGGATTCG	ACGAATCACACTTTTACCACT	55.0	38.1	58.1	55.5	935-956 bp	1.0	58.2
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	934-955 bp	1.0	57.6
trnS-GCU-trnG-UCC_p3	CACGGAAAGAGAGGGATTCG	AGAACGAATCACACTTTTACCAC	55.0	39.1	58.1	57.3	938-959 bp	1.0	57.2
trnS-GCU-trnG-UCC_p4	CACGGAAAGAGAGGGATTCG	GAACGAATCACACTTTTACCACT	55.0	39.1	58.1	57.3	937-958 bp	1.0	57.2
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.2	57.3	936-957 bp	1.0	56.5