Hotspot

rps16

LSC · 5083-6197 · 1115 bp

Back to Petrocosmea

Pi 0.0144

MarkerSeek score 59.60

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1115
variable_sites: 54
pi: 0.0144
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TACGAATCGAGCAAGAAGGG	AAACATGCTGCTTATTGGCG	50.0	45.0	57.4	57.7	1707-1740 bp	0.875	73.8
rps16_p2	GGATTCGACGGAAGGAATGA	AAACATGCTGCTTATTGGCG	50.0	45.0	57.4	57.7	1735-1768 bp	0.875	73.6
rps16_p3	CGGATTCGACGGAAGGAATG	AAACATGCTGCTTATTGGCG	55.0	45.0	58.8	57.7	1736-1769 bp	0.875	72.0
rps16_p4	TACGAATCGAGCAAGAAGGG	AACATGCTGCTTATTGGCGA	50.0	45.0	57.4	58.5	1706-1739 bp	0.875	71.7
rps16_p5	TACGAATCGAGCAAGAAGGG	ACATGCTGCTTATTGGCGAA	50.0	45.0	57.4	58.5	1705-1738 bp	0.875	71.7