Hotspot

petD

LSC · 77372-78612 · 1241 bp

Back to Petrocosmea

Pi 0.0143

MarkerSeek score 58.80

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1241
variable_sites: 47
pi: 0.0143
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TATTGCGCGGAAATGCTAGT	GAAGCCTCCCGGAATTTGAT	45.0	50.0	58.1	57.9	2063-2087 bp	1.0	83.0
petD_p2	GCCGCTTCTTACTGCTGTAT	GAAGCCTCCCGGAATTTGAT	50.0	50.0	58.1	57.9	1993-2017 bp	1.0	83.0
petD_p3	TATCGTCCGACCGTTACTGA	GAAGCCTCCCGGAATTTGAT	50.0	50.0	58.0	57.9	2394-2418 bp	1.0	82.9
petD_p4	ACTATCGTCCGACCGTTACT	GAAGCCTCCCGGAATTTGAT	50.0	50.0	58.0	57.9	2396-2420 bp	1.0	82.8
petD_p5	GGTTACAGGTGTGGTTCTGG	GAAGCCTCCCGGAATTTGAT	55.0	50.0	58.1	57.9	2212-2236 bp	1.0	82.4