Hotspot

rps15-ycf1

SSC · 122924-123292 · 369 bp

Back to Petrocosmea

Pi 0.0316

MarkerSeek score 66.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 369
variable_sites: 36
pi: 0.0316
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AACACCATGCATTGATGGGT	AGACCCCATTCATTTGCCTG	45.0	50.0	58.1	58.1	1324-1355 bp	1.0	86.1
rps15-ycf1_p2	TCCCAACGCGTTACATAAGG	AGACCCCATTCATTTGCCTG	50.0	50.0	58.0	58.1	1461-1492 bp	1.0	86.0
rps15-ycf1_p3	AACACCATGCATTGATGGGT	ATTTGCCTGGAAACGACGAT	45.0	45.0	58.1	58.2	1313-1344 bp	1.0	85.8
rps15-ycf1_p4	TCCCAACGCGTTACATAAGG	ATTTGCCTGGAAACGACGAT	50.0	45.0	58.0	58.2	1450-1481 bp	1.0	85.7
rps15-ycf1_p5	TCCCAACGCGTTACATAAGG	CTGGAAACGACGATGGACAA	50.0	50.0	58.0	58.2	1444-1475 bp	1.0	85.5