Hotspot

ccsA-ndhD

SSC · 114915-115156 · 242 bp

Back to Petrocosmea

Pi 0.0406

MarkerSeek score 73.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 242
variable_sites: 26
pi: 0.0406
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTCAGCAATTGTCGCGTCTA	GCATTACCAGGTCTGAGTGG	45.0	55.0	57.9	58.0	695-719 bp	1.0	86.0
ccsA-ndhD_p2	TTCAGCAATTGTCGCGTCTA	TCAGTAGCTTTTCGATGGCC	45.0	50.0	57.9	58.0	721-745 bp	1.0	85.8
ccsA-ndhD_p3	TTCAGCAATTGTCGCGTCTA	CAGTAGCTTTTCGATGGCCT	45.0	50.0	57.9	58.0	720-744 bp	1.0	85.8
ccsA-ndhD_p4	TTGGAGTTGGGACCCAAAAG	GCATTACCAGGTCTGAGTGG	50.0	55.0	57.9	58.0	794-818 bp	1.0	85.2
ccsA-ndhD_p5	TTGGAGTTGGGACCCAAAAG	TCAGTAGCTTTTCGATGGCC	50.0	50.0	57.9	58.0	820-844 bp	1.0	85.1