Hotspot

ndhF-rpl32

SSC · 112383-112734 · 352 bp

Back to Petrocosmea

Pi 0.037
MarkerSeek score 74.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
352
variable_sites
34
pi
0.037
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.94
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAAGGAAAGGT TTTGATACAGAAGTACGTTTTT 50.0 27.3 57.0 52.0 431-880 bp 1.0 47.6
ndhF-rpl32_p2 CTGGAAGTGGAAGGAAAGGT TTTTGATACAGAAGTACGTTTT 50.0 27.3 57.0 52.0 432-881 bp 1.0 47.6
ndhF-rpl32_p3 CTGGAAGTGGAAGGAAAGGT TTTTTGATACAGAAGTACGTTT 50.0 27.3 57.0 52.0 433-882 bp 1.0 47.6
ndhF-rpl32_p4 CTGGAAGTGGAAGGAAAGGT TTTTGATACAGAAGTACGTTTTT 50.0 26.1 57.0 52.9 432-881 bp 1.0 47.6
ndhF-rpl32_p5 CTGGAAGTGGAAGGAAAGGT TTTTTGATACAGAAGTACGTTTT 50.0 26.1 57.0 52.9 433-882 bp 1.0 47.6