Hotspot

psbE-petL

LSC · 65881-66787 · 907 bp

Back to Petrocosmea

Pi 0.0119

MarkerSeek score 63.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 907
variable_sites: 27
pi: 0.0119
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGCCACTAGTAGAAAACCGA	50.0	45.0	57.8	55.9	811-978 bp	1.0	68.2
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	AGCCACTAGTAGAAAACCGA	55.0	45.0	58.2	55.9	806-973 bp	1.0	67.9
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	AGCCACTAGTAGAAAACCGA	50.0	45.0	58.4	55.9	804-971 bp	1.0	66.7
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AAGCCACTAGTAGAAAACCGA	50.0	42.9	57.8	56.7	812-979 bp	1.0	66.4
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGCCACTAGTAGAAAACCGAA	50.0	42.9	57.8	56.7	811-978 bp	1.0	66.4