Hotspot

atpF-atpH

LSC · 13374-13748 · 375 bp

Back to Petrocosmea

Pi 0.0174

MarkerSeek score 69.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 375
variable_sites: 21
pi: 0.0174
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTGGCCTAAAGAAACGA	AGGCAGAGGGAAAGATACGA	50.0	50.0	57.8	57.8	525-546 bp	1.0	81.8
atpF-atpH_p2	TTAAATCCGAAACTCCCGGC	AGGCAGAGGGAAAGATACGA	50.0	50.0	58.3	57.8	551-572 bp	1.0	80.9
atpF-atpH_p3	CCAGTGGCCTAAAGAAACGA	GAGGCAGAGGGAAAGATACG	50.0	55.0	57.8	57.5	526-547 bp	1.0	78.9
atpF-atpH_p4	CCAGTGGCCTAAAGAAACGA	GCAGAGGGAAAGATACGAGG	50.0	55.0	57.8	57.5	523-544 bp	1.0	78.9
atpF-atpH_p5	CCAGTGGCCTAAAGAAACGA	GGCAGAGGGAAAGATACGAG	50.0	55.0	57.8	57.5	524-545 bp	1.0	78.9