Hotspot

trnK-UUU-rps16

LSC · 4261-5082 · 822 bp

Back to Petrocosmea

Pi 0.0169

MarkerSeek score 63.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 822
variable_sites: 44
pi: 0.0169
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCCTTCCCGTATCAGGTACT	GCCGTACGAGGAGAAAACTT	55.0	50.0	57.9	57.9	1276-1484 bp	1.0	85.9
trnK-UUU-rps16_p2	CCCTTCCCGTATCAGGTACT	CAAAGTGTATTACGCGGGGA	55.0	50.0	57.9	58.0	1722-1930 bp	0.875	81.3
trnK-UUU-rps16_p3	CCCTTCCCGTATCAGGTACT	TCAAAGTGTATTACGCGGGG	55.0	50.0	57.9	58.0	1723-1931 bp	0.875	81.3
trnK-UUU-rps16_p4	GGGGTCTTCCAAACCATACC	CAAAGTGTATTACGCGGGGA	55.0	50.0	57.9	58.0	1456-1664 bp	0.875	81.1
trnK-UUU-rps16_p5	GGGGTCTTCCAAACCATACC	TCAAAGTGTATTACGCGGGG	55.0	50.0	57.9	58.0	1457-1665 bp	0.875	81.1