Hotspot

trnH-GUG-psbA

LSC · 76-403 · 328 bp

Back to Petrocosmea

Pi 0.0748

MarkerSeek score 77.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 328
variable_sites: 59
pi: 0.0748
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	ATCCACTTGGCTACATCCG	TCCCTCTAGATCTAGCTGCT	52.6	50.0	56.9	56.3	352-393 bp	1.0	61.9
trnH-GUG-psbA_p2	ATCCACTTGGCTACATCCGC	TCCCTCTAGATCTAGCTGCT	55.0	50.0	60.2	56.3	352-393 bp	1.0	61.2
trnH-GUG-psbA_p3	CCACTTGGCTACATCCGC	TCCCTCTAGATCTAGCTGCT	61.1	50.0	58.2	56.3	350-391 bp	1.0	61.2
trnH-GUG-psbA_p4	ATCCACTTGGCTACATCCG	TTCCCTCTAGATCTAGCTGCT	52.6	47.6	56.9	57.1	353-394 bp	1.0	60.1
trnH-GUG-psbA_p5	ATCCACTTGGCTACATCCGC	TTCCCTCTAGATCTAGCTGCT	55.0	47.6	60.2	57.1	353-394 bp	1.0	59.4