Hotspot

ccsA-ndhD

SSC · 114876-115126 · 251 bp

Back to Petrocodon

Pi 0.0239
MarkerSeek score 67.80
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
251
variable_sites
14
pi
0.0239
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGCGTCTATTGGATTTCTT CCCGTACTAGGTATTGGTATGT 40.0 45.5 54.7 56.5 440-450 bp 1.0 46.1
ccsA-ndhD_p2 TGGCGTCTATTGGATTTCTT CCCGTACTAGGTATTGGTATG 40.0 47.6 54.7 54.7 440-450 bp 1.0 46.1
ccsA-ndhD_p3 TGGCGTCTATTGGATTTCTTA CCCGTACTAGGTATTGGTATGT 38.1 45.5 54.7 56.5 440-450 bp 1.0 46.1
ccsA-ndhD_p4 TGGCGTCTATTGGATTTCTT CCCGTACTAGGTATTGGTATGTA 40.0 43.5 54.7 56.4 440-450 bp 1.0 46.1
ccsA-ndhD_p5 TGGCGTCTATTGGATTTCTTAT CCCGTACTAGGTATTGGTATGT 36.4 45.5 55.3 56.5 440-450 bp 1.0 46.1