Hotspot

rpl32-trnL-UAG

SSC · 112881-113746 · 866 bp

Back to Petrocodon

Pi 0.0177

MarkerSeek score 63.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 866
variable_sites: 36
pi: 0.0177
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GAAGGGGTATTGTGCATCGT	TTTAGGAGATGACATGCCGC	50.0	50.0	58.0	58.0	1052-1086 bp	1.0	85.2
rpl32-trnL-UAG_p2	GAAGGGGTATTGTGCATCGT	ATGCCCTTTCCGGATGAATC	50.0	50.0	58.0	58.0	1258-1292 bp	1.0	85.0
rpl32-trnL-UAG_p3	GAAGGGGTATTGTGCATCGT	GCCCTTTCCGGATGAATCAT	50.0	50.0	58.0	58.0	1256-1290 bp	1.0	85.0
rpl32-trnL-UAG_p4	GAAGGGGTATTGTGCATCGT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	997-1031 bp	1.0	84.9
rpl32-trnL-UAG_p5	GAAGGGGTATTGTGCATCGT	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.0	58.1	1468-1502 bp	1.0	83.9