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ndhF-rpl32

SSC · 111983-112697 · 715 bp

Back to Petrocodon

Pi 0.0193

MarkerSeek score 61.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 715
variable_sites: 30
pi: 0.0193
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CAAGCATTTGACGCAAGAGG	ACGATGCACAATACCCCTTC	50.0	50.0	58.0	58.0	1324-1403 bp	1.0	82.9
ndhF-rpl32_p2	GCAAGAGGTCGTGTAAACCA	ACGATGCACAATACCCCTTC	50.0	50.0	58.1	58.0	1312-1391 bp	1.0	82.2
ndhF-rpl32_p3	CAAGCATTTGACGCAAGAGG	AATTTTTCGAGTCAGCCCGA	50.0	45.0	58.0	57.8	1448-1530 bp	1.0	81.7
ndhF-rpl32_p4	CAAGCATTTGACGCAAGAGG	TTTTTCGAGTCAGCCCGATT	50.0	45.0	58.0	57.8	1446-1528 bp	1.0	81.7
ndhF-rpl32_p5	AATAGAGCAGCACACAACGT	ACGATGCACAATACCCCTTC	45.0	50.0	57.8	58.0	1498-1577 bp	0.8	73.2