Hotspot

ndhC-trnV-UAC

LSC · 50795-51848 · 1054 bp

Back to Petrocodon

Pi 0.016
MarkerSeek score 62.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1054
variable_sites
38
pi
0.016
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAATGGGAATAAGACTTG GCACGTTGGGTCTTTGAAAC 39.1 50.0 57.6 58.2 1214-1234 bp 1.0 54.5
ndhC-trnV-UAC_p2 TGCCAAAATGGGAATAAGACTTG AATGCACGTTGGGTCTTTGA 39.1 45.0 57.6 58.3 1217-1237 bp 1.0 53.5
ndhC-trnV-UAC_p3 TGCCAAAATGGGAATAAGACTTG ATGCACGTTGGGTCTTTGAA 39.1 45.0 57.6 58.3 1216-1236 bp 1.0 53.4
ndhC-trnV-UAC_p4 TGCCAAAATGGGAATAAGACT GCACGTTGGGTCTTTGAAAC 38.1 50.0 55.4 58.2 1214-1234 bp 1.0 53.2
ndhC-trnV-UAC_p5 GCCAAAATGGGAATAAGACTTGA GCACGTTGGGTCTTTGAAAC 39.1 50.0 57.3 58.2 1213-1233 bp 1.0 52.5