Hotspot

trnE-UUC-trnT-GGU

LSC · 30889-31576 · 688 bp

Back to Petrocodon

Pi 0.0118

MarkerSeek score 63.00

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 17
pi: 0.0118
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	533-830 bp	1.0	84.5
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	512-809 bp	1.0	84.0
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	509-806 bp	1.0	83.9
trnE-UUC-trnT-GGU_p4	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	530-827 bp	1.0	83.3
trnE-UUC-trnT-GGU_p5	CTCCGTGAAAGAGAGGTGTC	GAACCGATGACTTACGCCTT	55.0	50.0	57.6	58.0	490-787 bp	1.0	83.0