Hotspot

rpoB-trnC-GCA

LSC · 27164-28141 · 978 bp

Back to Petrocodon

Pi 0.0183

MarkerSeek score 63.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 978
variable_sites: 42
pi: 0.0183
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGGTAATAAATGCCAGGGCT	ATCCTAGATGCGAACGAGGA	50.0	50.0	58.0	58.0	1602-1627 bp	1.0	85.0
rpoB-trnC-GCA_p2	CGGTAATAAATGCCAGGGCT	TCCTAGATGCGAACGAGGAT	50.0	50.0	58.0	58.0	1601-1626 bp	1.0	85.0
rpoB-trnC-GCA_p3	GAAAATACCGGATCCCCACC	TCCTAGATGCGAACGAGGAT	55.0	50.0	58.0	58.0	1916-1941 bp	1.0	84.3
rpoB-trnC-GCA_p4	CGGTAATAAATGCCAGGGCT	CCGATTATCTCTGAGGGGGT	50.0	55.0	58.0	58.0	1914-1938 bp	0.4	58.0
rpoB-trnC-GCA_p5	GAAAATACCGGATCCCCACC	CCGATTATCTCTGAGGGGGT	55.0	55.0	58.0	58.0	2229-2253 bp	0.4	57.7