Hotspot

atpA-atpF

LSC · 12046-12109 · 64 bp

Back to Petrocodon

Pi 0.0783

MarkerSeek score 74.40

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 8
pi: 0.0783
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CACTTGAAGTACGGTACCGG	ACATGTTGGGAGCGATGAAA	55.0	45.0	58.0	57.8	198-206 bp	1.0	83.8
atpA-atpF_p2	CCACTTGAAGTACGGTACCG	ACATGTTGGGAGCGATGAAA	55.0	45.0	58.0	57.8	199-207 bp	1.0	83.8
atpA-atpF_p3	CAATACCATCGCCCACTTGA	ACATGTTGGGAGCGATGAAA	50.0	45.0	58.0	57.8	211-219 bp	1.0	83.3
atpA-atpF_p4	CACTTGAAGTACGGTACCGG	GACATGTTGGGAGCGATGAA	55.0	50.0	58.0	58.3	199-207 bp	1.0	83.3
atpA-atpF_p5	CCACTTGAAGTACGGTACCG	GACATGTTGGGAGCGATGAA	55.0	50.0	58.0	58.3	200-208 bp	1.0	83.3