Hotspot

trnS-GCU-trnG-UCC

LSC · 8537-9386 · 850 bp

Back to Petrocodon

Pi 0.0168
MarkerSeek score 63.10
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
850
variable_sites
32
pi
0.0168
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CCACTCAGCCATCTCTCC ACGAATCACACTTTTACCACT 61.1 38.1 56.4 55.5 863-912 bp 1.0 46.9
trnS-GCU-trnG-UCC_p2 CCACTCAGCCATCTCTCC AGAACGAATCACACTTTTACCAC 61.1 39.1 56.4 57.3 866-915 bp 1.0 46.9
trnS-GCU-trnG-UCC_p3 CCACTCAGCCATCTCTCC GAACGAATCACACTTTTACCACT 61.1 39.1 56.4 57.3 865-914 bp 1.0 46.9
trnS-GCU-trnG-UCC_p4 CCACTCAGCCATCTCTCC AGGGTTAAAAGAACGAATCACAC 61.1 39.1 56.4 57.3 875-924 bp 1.0 46.8
trnS-GCU-trnG-UCC_p5 CCACTCAGCCATCTCTCC GGGTTAAAAGAACGAATCACACT 61.1 39.1 56.4 57.3 874-923 bp 1.0 46.8