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psbI-trnS-GCU

LSC · 8333-8448 · 116 bp

Back to Petrocodon

Pi 0.0491

MarkerSeek score 77.30

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 116
variable_sites: 13
pi: 0.0491
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	TTATTCGTACCGAGGGTTCG	50.0	50.0	57.1	57.2	176-179 bp	1.0	71.6
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	TTCGAATCCCTCTCTTTCCG	50.0	50.0	57.1	57.0	160-163 bp	1.0	71.3
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGA	GGGTTCGAATCCCTCTCTTT	50.0	50.0	57.1	56.9	163-166 bp	1.0	70.2
psbI-trnS-GCU_p4	CGTAATCCTGGACGTGAAGA	GGGTTCGAATCCCTCTCTTTC	50.0	52.4	57.1	58.1	163-166 bp	1.0	70.2
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGA	GGTTCGAATCCCTCTCTTTCC	50.0	52.4	57.1	58.1	162-165 bp	1.0	70.2