Hotspot

trnK-UUU-rps16

LSC · 4265-5070 · 806 bp

Back to Petrocodon

Pi 0.019

MarkerSeek score 58.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 806
variable_sites: 35
pi: 0.019
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	GTCGGGGTCTTCCAAACTTT	GCCGTACGAGGAGAAAACTT	50.0	50.0	58.0	57.9	1201-1207 bp	1.0	82.3
trnK-UUU-rps16_p2	GTCGGGGTCTTCCAAACTTT	TCTATCCCAATGAGCCGTCT	50.0	50.0	58.0	57.9	1145-1151 bp	1.0	82.2
trnK-UUU-rps16_p3	GTCGGGGTCTTCCAAACTTT	AGCCGTCTATCGAATCGTTG	50.0	50.0	58.0	57.9	1133-1139 bp	1.0	81.9
trnK-UUU-rps16_p4	GTCGGGGTCTTCCAAACTTT	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.0	57.9	1103-1109 bp	1.0	81.9
trnK-UUU-rps16_p5	GTCGGGGTCTTCCAAACTTT	CCGTCTATCGAATCGTTGCA	50.0	50.0	58.0	58.2	1131-1137 bp	1.0	81.6