Hotspot

rpl32

SSC · 110959-111123 · 165 bp

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Pi 0.497

MarkerSeek score 76.50

Primer available yes

Label rpl32

Feature Metadata

label_name: rpl32
feature_type: gene
region: SSC
length_bp: 165
variable_sites: 74
pi: 0.497
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32_p1	CCAATTTTCACGGATCTTTGACA	ATATCTGCCCTTCCCGAGAA	39.1	50.0	57.9	57.9	586 bp	0.5	34.2
rpl32_p2	CCAATTTTCACGGATCTTTGACA	TATCTGCCCTTCCCGAGAAA	39.1	50.0	57.9	58.1	585 bp	0.5	34.0
rpl32_p3	CCAATTTTCACGGATCTTTGACA	TGCCCTTCCCGAGAAAGATA	39.1	50.0	57.9	58.1	581 bp	0.5	34.0
rpl32_p4	GGGCTAAAAAGAGTACTTGATGC	ATATCTGCCCTTCCCGAGAA	43.5	50.0	57.8	57.9	1174 bp	0.5	33.3
rpl32_p5	GGGCTAAAAAGAGTACTTGATGC	TGCCCTTCCCGAGAAAGATA	43.5	50.0	57.8	58.1	1169 bp	0.5	33.1