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ndhG

SSC · 115989-116519 · 531 bp

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Pi 0.453

MarkerSeek score 78.40

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 196
pi: 0.453
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	CTTCTGGATTGACAGTGCCA	TTGATCACATCCGAGCGTTT	50.0	45.0	57.8	57.9	1898 bp	0.5	57.7
ndhG_p2	ACTTCTGGATTGACAGTGCC	TTGATCACATCCGAGCGTTT	50.0	45.0	57.8	57.9	1899 bp	0.5	57.7
ndhG_p3	ATGATCATACCTTGCGTGGC	TTGATCACATCCGAGCGTTT	50.0	45.0	58.4	57.9	1847 bp	0.5	56.0
ndhG_p4	CTTCTGGATTGACAGTGCCA	AATTGATCACATCCGAGCGT	50.0	45.0	57.8	57.7	1900 bp	0.5	56.0
ndhG_p5	CTTCTGGATTGACAGTGCCA	ATTGATCACATCCGAGCGTT	50.0	45.0	57.8	57.7	1899 bp	0.5	56.0