Hotspot

ndhD

SSC · 113357-114877 · 1521 bp

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Pi 0.464

MarkerSeek score 75.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1521
variable_sites: 477
pi: 0.464
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GGGCTCATATTGGAGTTGGG	TCTGCTCCAAGAACAGAGGA	55.0	50.0	58.0	58.0	2229 bp	0.5	59.8
ndhD_p2	GGGGCTCATATTGGAGTTGG	TCTGCTCCAAGAACAGAGGA	55.0	50.0	58.0	58.0	2230 bp	0.5	59.8
ndhD_p3	AGTGATTCACGTGGTTCTCG	TCTGCTCCAAGAACAGAGGA	50.0	50.0	57.9	58.0	1905 bp	0.5	59.3
ndhD_p4	TCGTGTGATTAGCCTAGGGT	TCTGCTCCAAGAACAGAGGA	50.0	50.0	57.8	58.0	2304 bp	0.5	58.6
ndhD_p5	GGGCTCATATTGGAGTTGGG	CAGAGGATTGTGTCGGTTGT	55.0	50.0	58.0	57.8	2216 bp	0.5	58.5