Hotspot

ccsA

SSC · 112124-113092 · 969 bp

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Pi 0.459

MarkerSeek score 77.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 333
pi: 0.459
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.0	58.0	1564 bp	0.5	59.6
ccsA_p2	TGAAATCGGTAGACACGCTG	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.0	58.0	1563 bp	0.5	59.6
ccsA_p3	GTGAAATCGGTAGACACGCT	CGAGAACCACGTGAATCACT	50.0	50.0	58.0	57.9	1277 bp	0.5	59.4
ccsA_p4	TGAAATCGGTAGACACGCTG	CGAGAACCACGTGAATCACT	50.0	50.0	58.0	57.9	1276 bp	0.5	59.4
ccsA_p5	GTGAAATCGGTAGACACGCT	GCATTACCGGGTATGAGTGG	50.0	55.0	58.0	58.1	1765 bp	0.5	59.0