Hotspot

ndhH-rps15

SSC · 120805-120895 · 91 bp

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Pi 0.446

MarkerSeek score 76.30

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 91
variable_sites: 37
pi: 0.446
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	TGAGGTAAATCAGCAGCGAC	GGCAGTTGGGTATTCGAGAG	50.0	55.0	58.0	58.1	629 bp	0.5	59.4
ndhH-rps15_p2	CCACCAAGAATGCCTACTCC	GGCAGTTGGGTATTCGAGAG	55.0	55.0	58.0	58.1	755 bp	0.5	59.2
ndhH-rps15_p3	AACACCATGCATTGATGGGT	GGCAGTTGGGTATTCGAGAG	45.0	55.0	58.1	58.1	198 bp	0.5	59.1
ndhH-rps15_p4	TGAGGTAAATCAGCAGCGAC	GGGAGCCGTTTGAGTATGTT	50.0	50.0	58.0	57.9	1121 bp	0.5	59.0
ndhH-rps15_p5	CCTAATTGCTCTGGCCCATT	GGCAGTTGGGTATTCGAGAG	50.0	55.0	57.9	58.1	392 bp	0.5	58.9