Hotspot

ndhG-ndhI

SSC · 116520-116873 · 354 bp

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Pi 0.5

MarkerSeek score 77.20

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 354
variable_sites: 159
pi: 0.5
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AGACCACGGAAGTTACTCCA	GATGGACTTTACCGAGGCTG	50.0	55.0	58.0	58.1	1362 bp	0.5	59.5
ndhG-ndhI_p2	AGACTTGTACAGACCACGGA	GATGGACTTTACCGAGGCTG	50.0	55.0	58.0	58.1	1372 bp	0.5	59.5
ndhG-ndhI_p3	AGACCACGGAAGTTACTCCA	TTGATCACATCCGAGCGTTT	50.0	45.0	58.0	57.9	1027 bp	0.5	59.2
ndhG-ndhI_p4	AGACTTGTACAGACCACGGA	TTGATCACATCCGAGCGTTT	50.0	45.0	58.0	57.9	1037 bp	0.5	59.2
ndhG-ndhI_p5	AGACCACGGAAGTTACTCCA	AGATGGACTTTACCGAGGCT	50.0	50.0	58.0	58.1	1363 bp	0.5	58.9