Hotspot

rps16

LSC · 6524-7625 · 1102 bp

Back to Pertya

Pi 0.00635
MarkerSeek score 62.40
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1102
variable_sites
7
pi
0.00635
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 AGAAATCACTATCCTACCCTTAGT TCTCTGTACCGACTGAACCA 37.5 50.0 56.1 57.7 1242-1266 bp 1.0 40.4
rps16_p2 AGAAATCACTATCCTACCCT TCTCTGTACCGACTGAACCA 40.0 50.0 52.0 57.7 1242-1266 bp 1.0 40.4
rps16_p3 AGAAATCACTATCCTACCCTT TCTCTGTACCGACTGAACCA 38.1 50.0 53.0 57.7 1242-1266 bp 1.0 40.4
rps16_p4 AGAAATCACTATCCTACCCTTAGTT TCTCTGTACCGACTGAACCA 36.0 50.0 56.8 57.7 1242-1266 bp 1.0 40.4
rps16_p5 AGAAATCACTATCCTACCCTTAGT GTCTCTGTACCGACTGAACC 37.5 55.0 56.1 57.4 1243-1267 bp 1.0 40.4