Hotspot

ccsA-ndhD

SSC · 115679-115901 · 223 bp

Back to Pertya

Pi 0.0404
MarkerSeek score 68.40
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
223
variable_sites
9
pi
0.0404
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGATATGCTATTTTGGGGTCA TCAGTTGACAAGGTTGAAGGT 40.9 42.9 57.2 57.4 349-378 bp 1.0 46.4
ccsA-ndhD_p2 TGGATATGCTATTTTGGGGTCAA TCAGTTGACAAGGTTGAAGGT 39.1 42.9 57.9 57.4 349-378 bp 1.0 43.7
ccsA-ndhD_p3 TTGGATATGCTATTTTGGGGTCA TCAGTTGACAAGGTTGAAGGT 39.1 42.9 57.9 57.4 350-379 bp 1.0 43.7
ccsA-ndhD_p4 TGGATATGCTATTTTGGGGTCA ATCAGTTGACAAGGTTGAAGGT 40.9 40.9 57.2 57.8 350-379 bp 1.0 42.2
ccsA-ndhD_p5 TGGATATGCTATTTTGGGGTCA CAGTTGACAAGGTTGAAGGT 40.9 45.0 57.2 55.8 348-377 bp 1.0 42.0