Hotspot

ndhF-rpl32

SSC · 112398-113454 · 1057 bp

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Pi 0.0132
MarkerSeek score 66.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1057
variable_sites
14
pi
0.0132
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGTCGGAAAAAGAAG CCGCCCAATATCCTTTCCTT 50.0 50.0 58.0 57.6 1237-1244 bp 1.0 77.7
ndhF-rpl32_p2 TGCCGTCGGAAAAAGAAGAA CCGCCCAATATCCTTTCCTT 45.0 50.0 58.0 57.6 1235-1242 bp 1.0 77.6
ndhF-rpl32_p3 TTGCCGTCGGAAAAAGAAGA CCGCCCAATATCCTTTCCTT 45.0 50.0 58.0 57.6 1236-1243 bp 1.0 77.6
ndhF-rpl32_p4 GTTGCCGTCGGAAAAAGAAG TGACAAAGCTTTTAACGCCG 50.0 45.0 58.0 57.6 1254-1261 bp 1.0 77.5
ndhF-rpl32_p5 TGCCGTCGGAAAAAGAAGAA TGACAAAGCTTTTAACGCCG 45.0 45.0 58.0 57.6 1252-1259 bp 1.0 77.4