Hotspot

rbcL-accD

LSC · 58003-58582 · 580 bp

Back to Pertya

Pi 0.00607
MarkerSeek score 63.80
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
580
variable_sites
3
pi
0.00607
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GAGTCCTGAACTAGCTGCTG AGCCTACATCTGTGTTCGAAC 55.0 47.6 57.7 58.0 686-769 bp 1.0 73.7
rbcL-accD_p2 GAGTCCTGAACTAGCTGCTG GCCTACATCTGTGTTCGAACT 55.0 47.6 57.7 58.0 685-768 bp 1.0 73.7
rbcL-accD_p3 GCTTGCAAATGGAGTCCTGA AGCCTACATCTGTGTTCGAAC 50.0 47.6 58.5 58.0 697-780 bp 1.0 72.3
rbcL-accD_p4 GCTTGCAAATGGAGTCCTGA GCCTACATCTGTGTTCGAACT 50.0 47.6 58.5 58.0 696-779 bp 1.0 72.3
rbcL-accD_p5 TGAGGCTTGCAAATGGAGTC GCCTACATCTGTGTTCGAACT 50.0 47.6 58.5 58.0 700-783 bp 1.0 72.3