Hotspot

ndhC-trnV-UAC

LSC · 51658-52812 · 1155 bp

Back to Pertya

Pi 0.0324
MarkerSeek score 78.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1155
variable_sites
37
pi
0.0324
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGCTAATCGGAGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1300-1329 bp 1.0 79.0
ndhC-trnV-UAC_p2 CGGTCCTTTGCTAATCGGAG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.4 57.8 1302-1331 bp 1.0 78.1
ndhC-trnV-UAC_p3 CTCCGGTCCTTTGCTAATCG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.4 57.8 1305-1334 bp 1.0 78.1
ndhC-trnV-UAC_p4 GTCCTTTGCTAATCGGAGCT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.7 57.5 1299-1328 bp 1.0 76.2
ndhC-trnV-UAC_p5 GGTCCTTTGCTAATCGGAGC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.7 57.8 1301-1330 bp 1.0 75.9