Hotspot

atpI-atpH

LSC · 26446-27624 · 1179 bp

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Pi 0.00869
MarkerSeek score 66.00
Primer available yes
Label atpI-atpH

Feature Metadata

label_name
atpI-atpH
feature_type
igs
region
LSC
length_bp
1179
variable_sites
10
pi
0.00869
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-atpH_p1 TCCCTGTCATGTTCCTTGGA ATAACGGAAGCGGCAGAAAT 50.0 45.0 58.3 58.0 1315-1348 bp 1.0 79.9
atpI-atpH_p2 TCCCTGTCATGTTCCTTGGA TACCTTGACCAACTCCAGGT 50.0 50.0 58.3 57.9 1371-1404 bp 1.0 79.2
atpI-atpH_p3 TCCCTGTCATGTTCCTTGGA AGCCAATCCAGCAGCAATAA 50.0 45.0 58.3 57.9 1331-1364 bp 1.0 79.0
atpI-atpH_p4 TCCCTGTCATGTTCCTTGGA AATAACGGAAGCGGCAGAAA 50.0 45.0 58.3 58.2 1316-1349 bp 1.0 78.7
atpI-atpH_p5 TCCCTGTCATGTTCCTTGGA GCAGTACCTTGACCAACTCC 50.0 55.0 58.3 58.2 1375-1408 bp 1.0 78.6