Hotspot

trnC-GCA-petN

LSC · 10778-11373 · 596 bp

Back to Pertya

Pi 0.0134
MarkerSeek score 65.60
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
596
variable_sites
8
pi
0.0134
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 662-896 bp 1.0 79.0
trnC-GCA-petN_p2 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 680-914 bp 1.0 76.7
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG GCCCAAGCGAGACTTACTAT 50.0 50.0 58.1 57.1 661-895 bp 1.0 73.0
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG ATTAAAGCAGCCCAAGCGAG 50.0 50.0 58.1 58.9 670-904 bp 1.0 72.9
trnC-GCA-petN_p5 ATTGTGTATCGTTTTGGCGG AGCCCAAGCGAGACTTACTA 45.0 50.0 57.1 58.2 719-953 bp 1.0 72.3