Hotspot

rpl32-trnL-UAG

SSC · 118606-119289 · 684 bp

Back to Persicaria

Pi 0.0931

MarkerSeek score 77.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 684
variable_sites: 240
pi: 0.0931
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCGAAATCTCTTTCTACCGGT	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	58.1	57.9	651-803 bp	0.923077	79.0
rpl32-trnL-UAG_p2	GCGAAATCTCTTTCTACCGGT	GTGGGTTGTTTCATTTGGGC	47.6	50.0	58.1	58.1	1293-1367 bp	0.692308	69.8
rpl32-trnL-UAG_p3	TGGCGAAATCTCTTTCTACCG	GTGGGTTGTTTCATTTGGGC	47.6	50.0	58.1	58.1	1295-1369 bp	0.615385	66.7
rpl32-trnL-UAG_p4	GCGAAATCTCTTTCTACCGGT	AGCAAAGCCCTGGGTAAAAA	47.6	45.0	58.1	57.9	1258-1330 bp	0.576923	64.0
rpl32-trnL-UAG_p5	TGGCGAAATCTCTTTCTACCG	AGCAAAGCCCTGGGTAAAAA	47.6	45.0	58.1	57.9	1260-1332 bp	0.576923	64.0