Hotspot

trnW-CCA-trnP-UGG

LSC · 66485-66810 · 326 bp

Back to Persicaria

Pi 0.0918

MarkerSeek score 62.10

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 326
variable_sites: 108
pi: 0.0918
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	307-474 bp	1.0	90.9
trnW-CCA-trnP-UGG_p2	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	50.0	50.0	58.0	58.1	308-475 bp	1.0	90.9
trnW-CCA-trnP-UGG_p3	GTTTTGGAGACCCACGTTCT	AGGGGAATGTTAACGCATCC	50.0	50.0	58.0	57.9	871-1045 bp	0.961538	89.7
trnW-CCA-trnP-UGG_p4	ACCTACGACATCGGGTTTTG	AGGGGAATGTTAACGCATCC	50.0	50.0	57.9	57.9	885-1059 bp	0.961538	89.3
trnW-CCA-trnP-UGG_p5	CCCCCTTTCCTGTCAAACAA	AGGGGAATGTTAACGCATCC	50.0	50.0	57.9	57.9	1159-1244 bp	0.653846	74.7