Hotspot

petA-psbJ

LSC · 63665-64399 · 735 bp

Back to Persicaria

Pi 0.0467

MarkerSeek score 83.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 145
pi: 0.0467
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TCGAGAAAGTTCAATTGTCCGA	ATAGGTACTGTAGCCGGCAT	40.9	50.0	58.0	58.0	731-884 bp	0.961538	74.3
petA-psbJ_p2	TCGAGAAAGTTCAATTGTCCGA	TAGCCGGCATTCTTGTGATC	40.9	50.0	58.0	58.0	721-874 bp	0.961538	74.0
petA-psbJ_p3	TCGAGAAAGTTCAATTGTCCGA	AATAGGTACTGTAGCCGGCA	40.9	50.0	58.0	58.2	732-885 bp	0.961538	72.6
petA-psbJ_p4	TCGAGAAAGTTCAATTGTCCGA	TAGGTACTGTAGCCGGCATT	40.9	50.0	58.0	58.2	730-883 bp	0.961538	72.6
petA-psbJ_p5	TCGAGAAAGTTCAATTGTCCGA	TACTGTAGCCGGCATTCTTG	40.9	50.0	58.0	57.7	726-879 bp	0.961538	71.9