Hotspot

ndhC-trnV-UAC

LSC · 51040-52107 · 1068 bp

Back to Persicaria

Pi 0.0594
MarkerSeek score 82.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1068
variable_sites
266
pi
0.0594
unique_haplotype_count
23
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ATCATATTCATAAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 26.1 50.0 52.2 57.8 1033-1225 bp 1.0 52.0
ndhC-trnV-UAC_p2 TCATATTCATAAAGCAGAAACAT AGAAGGTCTACGGTTCGAGT 26.1 50.0 52.2 57.8 1032-1224 bp 1.0 52.0
ndhC-trnV-UAC_p3 ATCATATTCATAAAGCAGAAACA GAAGGTCTACGGTTCGAGTC 26.1 55.0 52.2 57.5 1032-1224 bp 1.0 52.0
ndhC-trnV-UAC_p4 TCATATTCATAAAGCAGAAACAT GAAGGTCTACGGTTCGAGTC 26.1 55.0 52.2 57.5 1031-1223 bp 1.0 52.0
ndhC-trnV-UAC_p5 ATCATATTCATAAAGCAGAAACAT AGAAGGTCTACGGTTCGAGT 25.0 50.0 52.9 57.8 1033-1225 bp 1.0 52.0